16S and microbiome analysis guides
Clear, referenced explanations of the decisions behind a good amplicon analysis, from choosing a region to interpreting diversity.
16S rRNA Sequencing Data Analysis: A Complete Guide
How 16S rRNA amplicon data is analyzed, from raw FASTQ to taxonomy and diversity: primer trimming, DADA2 ASVs, SILVA classification, diversity statistics and reporting.
Read guide →V3-V4 vs V4 vs V1-V3: Choosing a 16S Region
Which 16S rRNA hypervariable region to sequence: V3-V4, V4, V1-V2 or V1-V3. Primers, read lengths needed to merge, taxonomic resolution and best use cases.
Read guide →ASVs vs OTUs: Why DADA2 Replaced OTU Clustering
The difference between amplicon sequence variants (ASVs) and operational taxonomic units (OTUs), why DADA2 ASVs are now the standard, and when OTUs still appear.
Read guide →How Much Does 16S Analysis Cost in Canada?
What 16S rRNA data analysis costs in Canada in 2026: per-sample platform pricing in CAD, what is included, and the real cost of doing it yourself.
Read guide →Analyze your 16S or ITS data today
Your first 3 analyses are free, with no credit card. Upload FASTQ files and get a publication-ready report, processed and stored in Canada.